Western Blot (WB)
(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)
Rabbit GALNT4 Polyclonal Antibody | anti-GALNT4 antibody
GALNT4 Antibody (N-term)
Western Blot (WB)
(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)
Western Blot (WB)
(GALNT4 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GALNT4 antibody detected the GALNT4 protein (arrow).)
Flow Cytometry (FC/FACS)
(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GALNT4 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
UDP-N-acetyl-alpha-D-galactosamine:polypeptide
N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes.
GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi
apparatus by catalyzing the transfer of GalNAc to serine and
threonine residues on target proteins. They are characterized by an
N-terminal transmembrane domain, a stem region, a lumenal catalytic
domain containing a GT1 motif and Gal/GalNAc transferase motif, and
a C-terminal ricin/lectin-like domain. GalNAc-Ts have different,
but overlapping, substrate specificities and patterns of
expression. In vitro, the encoded protein can complement other
GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem
repeat and can O-glycosylate the P-selectin glycoprotein ligand-1
molecule. The coding region of this gene is contained within a
single exon.
Argueso, P., et al. Invest. Ophthalmol. Vis. Sci. 44(1):86-92(2003)
Hassan, H., et al. J. Biol. Chem. 275(49):38197-38205(2000)
Bennett, E.P., et al. J. Biol. Chem. 273(46):30472-30481(1998)
Bennett, E.P., et al. Glycobiology 8(6):547-555(1998)
NCBI and Uniprot Product Information
NCBI Description
This locus represents naturally occurring transcripts that splice the 5' exons of the POC1B (POC1 centriolar protein homolog B) gene on chromosome 12 to the GALNT4 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4) gene, which is located within a POC1B intron. Alternative splicing results in two transcript variants, one of which encodes a fusion isoform that shares sequence identity with the products of each individual gene. [provided by RefSeq, Dec 2010]
Uniprot Description
GALNT4: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane.
Protein type: Transferase; Membrane protein, integral; EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis
Chromosomal Location of Human Ortholog: 12q21.33
Cellular Component: Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; integral to membrane
Molecular Function: metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity; carbohydrate binding
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; carbohydrate metabolic process; post-translational protein modification
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Product Notes
The GALNT4 galnt4 (Catalog #AAA9214580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 93-120. The GALNT4 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the GALNT4 galnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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