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Western Blot (WB) (Western blot analysis of ACSM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACSM1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human ACSM1 Polyclonal Antibody | anti-ACSM1 antibody

ACSM1 Antibody (N-term)

Gene Names
ACSM1; BUCS1; MACS1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACSM1; Polyclonal Antibody; ACSM1 Antibody (N-term); Acyl-coenzyme A synthetase ACSM1; mitochondrial; Acyl-CoA synthetase medium-chain family member 1; Butyrate--CoA ligase 1; Butyryl-coenzyme A synthetase 1; Lipoate-activating enzyme; Middle-chain acyl-CoA synthetase 1; BUCS1; LAE; MACS1; anti-ACSM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-48 amino acids from the N-terminal region of human ACSM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
21-48
Sequence Length
577
Applicable Applications for anti-ACSM1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ACSM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACSM1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ACSM1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACSM1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ACSM1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ACSM1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ACSM1 antibody
ACSM1 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy-and 2,3-or 3,4-unsaturated acids (in vitro). This protein functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase
Product Categories/Family for anti-ACSM1 antibody
References
Celis,J.E., et.al., Mol. Cell Proteomics 7 (10), 1795-1809 (2008)
Sullivan,P.F., et.al., Mol. Psychiatry 13 (6), 570-584 (2008)
Haketa,A., et.al., J. Hypertens. 22 (10), 1903-1907 (2004)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65273
NCBI Official Full Name
acyl-coenzyme A synthetase ACSM1, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA synthetase medium-chain family member 1
NCBI Official Symbol
ACSM1
NCBI Official Synonym Symbols
BUCS1; MACS1
NCBI Protein Information
acyl-coenzyme A synthetase ACSM1, mitochondrial
UniProt Protein Name
Acyl-coenzyme A synthetase ACSM1, mitochondrial
UniProt Gene Name
ACSM1
UniProt Synonym Gene Names
BUCS1; LAE; MACS1
UniProt Entry Name
ACSM1_HUMAN

Uniprot Description

ACSM1: Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4- unsaturated acids (in vitro). Functions as GTP-dependent lipoate- activating enzyme that generates the substrate for lipoyltransferase. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.2.1.2; Ligase; Carbohydrate Metabolism - butanoate; Mitochondrial

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: mitochondrial matrix

Molecular Function: acyl-CoA ligase activity; butyrate-CoA ligase activity; GTP binding; fatty-acid ligase activity; metal ion binding; ATP binding

Biological Process: cholesterol homeostasis; xenobiotic metabolic process; fatty acid oxidation; benzoate metabolic process; butyrate metabolic process; fatty acid biosynthetic process; energy derivation by oxidation of organic compounds

Research Articles on ACSM1

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Product Notes

The ACSM1 acsm1 (Catalog #AAA9214025) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-48. The ACSM1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSM1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the ACSM1 acsm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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