Western Blot (WB)
(Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).)
Rabbit anti-Human Rnasen Polyclonal Antibody | anti-Drosha antibody
Mouse Rnasen Antibody (Center)
Gene Names
Drosha; Rn3; Etohi2; Rnasen; AI874853; 1110013A17Rik
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Rnasen; Polyclonal Antibody; Mouse Rnasen Antibody (Center); Ribonuclease 3; Protein Drosha; Ribonuclease III; RNase III; Drosha; Etohi2; Rn3; anti-Drosha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
737-764
Sequence Length
1,373
Applicable Applications for anti-Drosha antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).)
Western Blot (WB)
(Mouse Rnasen Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Rnasen antibody detected the Rnasen protein (arrow).)
Related Product Information for anti-Drosha antibody
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).
References
Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010)
Yang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010)
Michon, F., et al. Dev. Biol. 340(2):355-368(2010)
Wu, H., et al. PLoS ONE 4 (10), E7566 (2009) :
Shenoy, A., et al. PLoS ONE 4 (9), E6971 (2009) :
Yang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010)
Michon, F., et al. Dev. Biol. 340(2):355-368(2010)
Wu, H., et al. PLoS ONE 4 (10), E7566 (2009) :
Shenoy, A., et al. PLoS ONE 4 (9), E6971 (2009) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
158828
NCBI Official Full Name
ribonuclease 3
NCBI Official Synonym Full Names
drosha, ribonuclease type III
NCBI Official Symbol
Drosha
NCBI Official Synonym Symbols
Rn3; Etohi2; Rnasen; AI874853; 1110013A17Rik
NCBI Protein Information
ribonuclease 3
UniProt Protein Name
Ribonuclease 3
UniProt Gene Name
Drosha
UniProt Synonym Gene Names
Etohi2; Rn3; Rnasen; RNase III
UniProt Entry Name
RNC_MOUSE
Uniprot Description
DROSHA: a nuclear type-III RNase that, along with the DGCR8, executes the initial step of microRNA (miRNA) processing in the nucleus. Cleaves the local hairpin structures embedded in long primary microRNA transcripts (pri-miRNAs) into pre-miRNAs. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Interacts with Sp1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ribonuclease; EC 3.1.26.3; RNA processing; Nucleolus; RNA-binding
Cellular Component: nucleus
Molecular Function: endoribonuclease activity; protein binding; nuclease activity; protein homodimerization activity; ribonuclease III activity; hydrolase activity; RNA binding; lipopolysaccharide binding; endonuclease activity; metal ion binding
Biological Process: RNA processing; defense response to Gram-positive bacterium; regulation of gene expression; pre-microRNA processing; rRNA catabolic process; ribosome biogenesis and assembly; primary microRNA processing; defense response to Gram-negative bacterium
Research Articles on Drosha
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Product Notes
The Drosha drosha (Catalog #AAA9213800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 737-764. The Mouse Rnasen Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rnasen can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Drosha drosha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rnasen, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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