Rabbit GNB2L1 Polyclonal Antibody | anti-GNB2L1 antibody

GNB2L1 Antibody (N-term)

Cat. Num. AAA9212879

• Gene Names: GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Monkey, Mouse, Pig, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GNB2L1; Polyclonal Antibody; GNB2L1 Antibody (N-term); Guanine nucleotide-binding protein subunit beta-2-like 1; Cell proliferation-inducing gene 21 protein; Guanine nucleotide-binding protein subunit beta-like protein 123; Human lung cancer oncogene 7 protein; HLC-7; Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1; N-terminally processed; anti-GNB2L1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Monkey, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human GNB2L1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 29-58
• Sequence Length: 317

• Applicable Applications for anti-GNB2L1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-GNB2L1 Pab is used in Western blot to detect GNB2L1 in Ramos cell lysate.)

Western Blot (WB)

(Western blot analysis of GNB2L1 (arrow) using GNB2L1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNB2L1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-GNB2L1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-GNB2L1 antibody

Cell Biology; Signal transduction

References

Ceci, M., et al., Nature 426(6966):579-584 (2003).
Usacheva, A., et al., J. Immunol. 171(6):2989-2994 (2003).
Kiely, P.A., et al., J. Biol. Chem. 277(25):22581-22589 (2002).
Tcherkasowa, A.E., et al., J. Immunol. 169(9):5161-5170 (2002).
Liedtke, C.M., et al., J. Biol. Chem. 277(25):22925-22933 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 35077
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-2-like 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• NCBI Official Symbol: GNB2L1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Gene Name: GNB2L1
• UniProt Synonym Gene Names: HLC-7; RACK1
• UniProt Entry Name: GBLP_HUMAN

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric beta; G protein; Motility/polarity/chemotaxis; Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: small ribosomal subunit; cytoskeleton; mitochondrion; cell soma; perinuclear region of cytoplasm; dendrite; cytoplasm; perikaryon; midbody; cytosol; nucleus; phagocytic cup

Molecular Function: protein tyrosine kinase inhibitor activity; protein binding; enzyme binding; protein homodimerization activity; protein kinase C binding; caspase activator activity; protein complex scaffold; SH2 domain binding; receptor tyrosine kinase binding; protein phosphatase binding; ion channel inhibitor activity; receptor binding

Biological Process: caspase activation; negative regulation of phagocytosis; negative regulation of protein kinase B signaling cascade; negative regulation of Wnt receptor signaling pathway; viral reproduction; positive regulation of apoptosis; regulation of cell cycle; positive regulation of protein homooligomerization; rhythmic process; gastrulation; cell cycle; osteoblast differentiation; regulation of protein localization; positive regulation of mitochondrial depolarization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of translation; positive regulation of cAMP catabolic process; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; regulation of cell division; positive regulation of GTPase activity; positive regulation of cell migration