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Western Blot (WB) (LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Rabbit anti-Human LSP1 Polyclonal Antibody | anti-LSP1 antibody

LSP1 Antibody (Center)

Gene Names
LSP1; WP34; pp52
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LSP1; Polyclonal Antibody; LSP1 Antibody (Center); Lymphocyte-specific protein 1; 47 kDa actin-binding protein; 52 kDa phosphoprotein; pp52; Lymphocyte-specific antigen WP34; WP34; anti-LSP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human LSP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
108-134
Sequence Length
467
Applicable Applications for anti-LSP1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Western Blot (WB) (LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).)

Immunohistochemistry (IHC)

(LSP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LSP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (LSP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LSP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LSP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (LSP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LSP1 antibody
LSP1 is an intracellular F-actin binding
protein. The protein is expressed in lymphocytes, neutrophils,
macrophages, and endothelium and may regulate neutrophil motility,
adhesion to fibrinogen matrix proteins, and transendothelial
migration. Alternative splicing results in multiple transcript
variants encoding different isoforms.
Product Categories/Family for anti-LSP1 antibody
References
Davila, S., et al. Genes Immun. (2010)
Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010)
Tamimi, R.M., et al. Cancer Causes Control (2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37192
NCBI Official Full Name
lymphocyte-specific protein 1 isoform 2
NCBI Official Synonym Full Names
lymphocyte-specific protein 1
NCBI Official Symbol
LSP1
NCBI Official Synonym Symbols
WP34; pp52
NCBI Protein Information
lymphocyte-specific protein 1
UniProt Protein Name
Lymphocyte-specific protein 1
UniProt Gene Name
LSP1
UniProt Synonym Gene Names
WP34; pp52
UniProt Entry Name
LSP1_HUMAN

NCBI Description

This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LSP1: May play a role in mediating neutrophil activation and chemotaxis. Binds actin. Activated T-lymphocytes.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; plasma membrane; actin cytoskeleton

Molecular Function: signal transducer activity; actin binding

Biological Process: cellular defense response; cell motility; chemotaxis; signal transduction

Research Articles on LSP1

Similar Products

Product Notes

The LSP1 lsp1 (Catalog #AAA9212241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 108-134. The LSP1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LSP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the LSP1 lsp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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