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Western Blot (WB) (All lanes : Anti-PECI Antibody at 1:1000 dilutionLane 1: H.liver tissue lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human PECI Polyclonal Antibody | anti-ECI2 antibody

PECI Antibody

Gene Names
ECI2; DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PECI; Polyclonal Antibody; PECI Antibody; Enoyl-CoA delta isomerase 2; mitochondrial; DRS-1; Delta(3); delta(2)-enoyl-CoA isomerase; D3; D2-enoyl-CoA isomerase; Diazepam-binding inhibitor-related protein 1; DBI-related protein 1; Dodecenoyl-CoA isomerase; Hepatocellular carcinoma-associated antigen 88; Peroxisomal 3; 2-trans-enoyl-CoA isomerase; pECI; Renal carcinoma antigen NY-REN-1; ECI2; DRS1; HCA88; anti-ECI2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
51-110
Sequence Length
359
Applicable Applications for anti-ECI2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)-Paraffin
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human PECI
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-PECI Antibody at 1:1000 dilutionLane 1: H.liver tissue lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PECI Antibody at 1:1000 dilutionLane 1: H.liver tissue lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ECI2 antibody
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates (By similarity).
Product Categories/Family for anti-ECI2 antibody
References
Suk K.,et al.Biochim. Biophys. Acta 1454:126-131(1999).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Geisbrecht B.V.,et al.J. Biol. Chem. 274:21797-21803(1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43585
NCBI Official Full Name
enoyl-CoA delta isomerase 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
enoyl-CoA delta isomerase 2
NCBI Official Symbol
ECI2
NCBI Official Synonym Symbols
DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
NCBI Protein Information
enoyl-CoA delta isomerase 2, mitochondrial
UniProt Protein Name
Enoyl-CoA delta isomerase 2, mitochondrial
Protein Family
UniProt Gene Name
ECI2
UniProt Synonym Gene Names
DRS1; HCA88; PECI; D3,D2-enoyl-CoA isomerase; DBI-related protein 1; pECI
UniProt Entry Name
ECI2_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]

Uniprot Description

PECI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.3.3.8; Isomerase; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: nucleoplasm; peroxisomal matrix; mitochondrion; intracellular membrane-bound organelle; membrane

Molecular Function: acyl-CoA binding; dodecenoyl-CoA delta-isomerase activity; receptor binding

Biological Process: metabolic process; fatty acid catabolic process

Research Articles on ECI2

Similar Products

Product Notes

The ECI2 eci2 (Catalog #AAA9211182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 51-110. The PECI Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PECI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)-Paraffin. WB~~ 1:1000. Researchers should empirically determine the suitability of the ECI2 eci2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PECI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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