Western Blot (WB)
(Western blot analysis of anti-Phospho-STAT5 Pab in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab.)
Rabbit anti-Human Phospho-STAT5a (Y694) Polyclonal Antibody | anti-STAT5A antibody
Phospho-STAT5a (Y694) Antibody
Western Blot (WB)
(Western blot analysis of anti-Phospho-STAT5 Pab in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-Phospho-STAT5 Pab in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Fluorescent confocal image of an SY5Y cell stained with MBS9211000 Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with MBS9211000 Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min)(not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus.)
Immunofluorescence (IF)
(Fluorescent confocal image of an SY5Y cell stained with MBS9211000 Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with MBS9211000 Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min)(not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Defilippi, P., et al., J. Cell Biol. 168(7):1099-1108 (2005).
Sekine, Y., et al., J. Biol. Chem. 280(9):8188-8196 (2005).
Sultan, A.S., et al., Oncogene 24(5):746-760 (2005).
Moriggl, R., et al., Cancer Cell 7(1):87-99 (2005).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Uniprot Description
STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; cytosol
Molecular Function: signal transducer activity; protein binding; transcription factor activity
Biological Process: lactation; succinate metabolic process; peptidyl-tyrosine phosphorylation; oxaloacetate metabolic process; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; positive regulation of multicellular organism growth; female pregnancy; positive regulation of mitotic cell cycle; positive regulation of activated T cell proliferation; fatty acid metabolic process; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; allantoin metabolic process; negative regulation of mast cell apoptosis; natural killer cell differentiation; luteinization; regulation of steroid metabolic process; development of secondary male sexual characteristics; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; regulation of multicellular organism growth; citrate metabolic process; valine metabolic process; development of secondary female sexual characteristics; positive regulation of natural killer cell mediated cytotoxicity; JAK-STAT cascade; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response
Research Articles on STAT5A
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Product Notes
The STAT5A stat5a (Catalog #AAA9211000) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-STAT5a (Y694) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-STAT5a (Y694) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the STAT5A stat5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-STAT5a (Y694), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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