Mouse STAT5A Monoclonal Antibody | anti-STAT5A antibody

Anti-STAT5A Antibody (clone 9F7) IHC-plus

Cat. Num. AAA249362

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Dog, Rat, Human, Monkey
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: STAT5A; Monoclonal Antibody; Anti-STAT5A Antibody (clone 9F7) IHC-plus; Mouse Monoclonal [clone 9F7] (IgG1) to Human STAT5A; Human STAT5A; MGF; STAT5; anti-STAT5A antibody

• Host: Mouse
• Reactivity: Dog, Rat, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9F7
• Specificity: Human STAT5A / STAT5
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.73 mg/ml (varies by lot)
• Sequence Length: 794

• Applicable Applications for anti-STAT5A antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:500 - 1:2000)
• Immunogen: STAT5A antibody was raised against full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-STAT5A antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-STAT5A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-STAT5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-STAT5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-STAT5A antibody

STAT5A Antibody, MGF Antibody, STAT5 Antibody Description: STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229; Q1KLZ6
• Molecular Weight: 86,949 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: protein binding; signal transducer activity; transcription factor activity

Biological Process: 2-oxoglutarate metabolic process; allantoin metabolic process; citrate metabolic process; creatine metabolic process; creatinine metabolic process; development of secondary female sexual characteristics; development of secondary male sexual characteristics; fatty acid metabolic process; female pregnancy; isoleucine metabolic process; JAK-STAT cascade; lactation; luteinization; natural killer cell differentiation; negative regulation of erythrocyte differentiation; negative regulation of mast cell apoptosis; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; Peyer's patch development; positive regulation of activated T cell proliferation; positive regulation of B cell differentiation; positive regulation of gamma-delta T cell differentiation; positive regulation of inflammatory response; positive regulation of interleukin-2 biosynthetic process; positive regulation of mitotic cell cycle; positive regulation of multicellular organism growth; positive regulation of natural killer cell differentiation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; regulation of multicellular organism growth; regulation of steroid metabolic process; regulation of transcription from RNA polymerase II promoter; sequestering of lipid; succinate metabolic process; T cell differentiation in the thymus; T cell homeostasis; taurine metabolic process; transcription, DNA-dependent; valine metabolic process