Rabbit ZIC1 Polyclonal Antibody | anti-ZIC1 antibody

ZIC1 Antibody (N-term)

Cat. Num. AAA9210127

• Gene Names: ZIC1; ZIC; ZNF201
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ZIC1; Polyclonal Antibody; ZIC1 Antibody (N-term); Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC; ZNF201; anti-ZIC1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ZIC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human ZIC1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.42 (varies by lot)
• Sequence Positions: 6-34
• Sequence Length: 447

• Applicable Applications for anti-ZIC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-ZIC1 Antibody (N-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(ZIC1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ZIC1 antibody detected the ZIC1 protein (arrow).)

Related Product Information for anti-ZIC1 antibody

This gene encodes a member of the ZIC family of C2H2-type
zinc finger proteins. Members of this family are important during
development. Aberrant expression of this gene is seen in
medulloblastoma, a childhood brain tumor. This gene is closely
linked to the gene encoding zinc finger protein of the cerebellum
4, a related family member on chromosome 3. This gene encodes a
transcription factor that can bind and transactivate the
apolipoprotein E gene.

Product Categories/Family for anti-ZIC1 antibody

Neuroscience

References

Brill, E., et al. Cancer Res. 70(17):6891-6901(2010)
Wang, L.J., et al. Biochem. Biophys. Res. Commun. 379(4):959-963(2009)
Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009)
Arora, A., et al. RNA 14(7):1290-1296(2008)
Pourebrahim, R., et al. FEBS Lett. 581(26):5122-5126(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 22547182
• NCBI GeneID: 7545
• NCBI Accession #: NP_003403.2
• NCBI GenBank Nucleotide #: NM_003412.3
• UniProt Accession #: Q15915; Q2M3N1
• Molecular Weight: 48309
• NCBI Official Full Name: zinc finger protein ZIC 1
• NCBI Official Synonym Full Names: Zic family member 1
• NCBI Official Symbol: ZIC1
• NCBI Official Synonym Symbols: ZIC; ZNF201
• NCBI Protein Information: zinc finger protein ZIC 1
• UniProt Protein Name: Zinc finger protein ZIC 1
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC1
• UniProt Synonym Gene Names: ZIC; ZNF201
• UniProt Entry Name: ZIC1_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZIC1: Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally- located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'- TGGGTGGTC-3'. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: inner ear morphogenesis; positive regulation of protein import into nucleus; transcription, DNA-dependent; spinal cord development; positive regulation of transcription, DNA-dependent; brain development; cell differentiation; pattern specification process; adult walking behavior; regulation of smoothened signaling pathway