Rabbit anti-Human RBMX Polyclonal Antibody | anti-RBMX antibody

RBMX Antibody (Center)

Cat. Num. AAA9209730

• Gene Names: RBMX; RNMX; HNRPG; HNRNPG; RBMXP1; RBMXRT; hnRNP-G
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RBMX; Polyclonal Antibody; RBMX Antibody (Center); RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G; N-terminally processed; HNRPG; RBMXP1; anti-RBMX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RBMX antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 222-252 amino acids from the Central region of human RBMX.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 196

• Applicable Applications for anti-RBMX antibody: Western Blot (WB)
• Application Notes: WB~~1:2000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RBMX Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: K562 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-RBMX Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: Hela whole cell lysatesLane 3: K562 whole cell lysatesLane 4: A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RBMX antibody

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single- stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1- beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.

Product Categories/Family for anti-RBMX antibody

Crown Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism; Neuroscience

References

Soulard M.,et al.Nucleic Acids Res. 21:4210-4217(1993).
Venables J.P.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Lingenfelter P.A.,et al.Mamm. Genome 12:538-545(2001).
Lin T.-Y.,et al.Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 258645150
• NCBI GeneID: 27316
• NCBI Accession #: NP_001158275.1
• NCBI GenBank Nucleotide #: NM_001164803.1
• UniProt Accession #: P38159; Q5JQ67; Q8N8Y7; Q969R3; B4E3U4; D3DWH0; E9PG86
• Molecular Weight: 42332
• NCBI Official Full Name: RNA-binding motif protein, X chromosome isoform 2
• NCBI Official Synonym Full Names: RNA binding motif protein, X-linked
• NCBI Official Symbol: RBMX
• NCBI Official Synonym Symbols: RNMX; HNRPG; HNRNPG; RBMXP1; RBMXRT; hnRNP-G
• NCBI Protein Information: RNA-binding motif protein, X chromosome
• UniProt Protein Name: RNA-binding motif protein, X chromosome
• Protein Family: RNA-binding motif protein
• UniProt Gene Name: RBMX
• UniProt Synonym Gene Names: HNRPG; RBMXP1; hnRNP G
• UniProt Entry Name: RBMX_HUMAN

NCBI Description

This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq, Sep 2009]

Uniprot Description

RBMX: RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single- stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1- beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding; Tumor suppressor; RNA processing

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: nucleoplasm; extracellular space; membrane; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of nuclear mRNA splicing, via spliceosome; osteoblast differentiation; nuclear mRNA splicing, via spliceosome; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; membrane protein ectodomain proteolysis; positive regulation of transcription from RNA polymerase II promoter; gene expression; protein homooligomerization; regulation of alternative nuclear mRNA splicing, via spliceosome