Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9209718 Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Rabbit Beclin 1 Polyclonal Antibody | anti-BECN1 antibody
Beclin 1 Antibody
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9209718 Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9209718 Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Western Blot (WB)
(Beclin 1 Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Beclin 1 antibody detected the Beclin 1 protein (arrow).)
Western Blot (WB)
(Beclin 1 Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the Beclin 1 antibody detected the Beclin 1 protein (arrow).)
1.Koukourakis, M.I., et al. Br. J. Cancer 103(8):1209-1214(2010)
2.Jaeger, P.A., et al. Arch. Neurol. 67(10):1181-1184(2010)
3. Metzger, S., et al. Hum. Genet. 128(4):453-459(2010)
4.Oberstein, A., et al. J. Biol. Chem. 282(17):13123-13132(2007)
5. Furuya, N., et al. Autophagy 1(1):46-52(2005)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
NCBI and Uniprot Product Information
NCBI Description
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]
Uniprot Description
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Protein type: Autophagy; Adaptor/scaffold; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; trans-Golgi network; phagocytic vesicle; nucleus; endosome
Molecular Function: protein binding; phosphoinositide 3-kinase binding
Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; regulation of catalytic activity; lysosome organization and biogenesis; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; positive regulation of macroautophagy; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation
Research Articles on BECN1
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Product Notes
The BECN1 becn1 (Catalog #AAA9209718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 100-123. The Beclin 1 Antibody reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin 1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:100 WB~~1:1000. Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beclin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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