Western Blot (WB)
(Western blot analysis of anti-PI4K II beta Pab in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, mouse PI 4 Kinase type 2 beta Polyclonal Antibody | anti-PI4K2B antibody
PI 4 Kinase type 2 beta antibody (C-term)
Western Blot (WB)
(Western blot analysis of anti-PI4K II beta Pab in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-PI4K II beta Pab in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Mora, S., et al., J. Biol. Chem. 277(30):27494-27500 (2002).
Balla, A., et al., J. Biol. Chem. 277(22):20041-20050 (2002).
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 [PubMed 12324459]).[supplied by OMIM, Jun 2008]
Uniprot Description
PI4K2B: Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
Protein type: EC 2.7.1.67; Motility/polarity/chemotaxis; Kinase, lipid
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: membrane; cytoplasm; cytosol
Molecular Function: 1-phosphatidylinositol 4-kinase activity; ATP binding
Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process
Research Articles on PI4K2B
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Product Notes
The PI4K2B pi4k2b (Catalog #AAA9209684) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 305-336. The PI 4 Kinase type 2 beta antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PI 4 Kinase type 2 beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PI4K2B pi4k2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI 4 Kinase type 2 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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