Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with DDIT3 Antibody (C-term A135). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). DDIT3 immunoreactivity is localized to Cytoplasm significantly.)
Rabbit DDIT3 Polyclonal Antibody | anti-DDIT3 antibody
DDIT3 Antibody (C-term A135)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with DDIT3 Antibody (C-term A135). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). DDIT3 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with DDIT3 Antibody (C-term A135). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). DDIT3 immunoreactivity is localized to Cytoplasm significantly.)
Western Blot (WB)
(DDIT3 Antibody (C-term A135) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).)
Western Blot (WB)
(DDIT3 Antibody (C-term A135) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).)
Flow Cytometry (FC/FACS)
(DDIT3 Antibody (C-term A135) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DDIT3 Antibody (C-term A135) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
protein (C/EBP) family of transcription factors. The protein
functions as a dominant-negative inhibitor by forming heterodimers
with other C/EBP members, such as C/EBP and LAP (liver activator
protein), and preventing their DNA binding activity. The protein is
implicated in adipogenesis and erythropoiesis, is activated by
endoplasmic reticulum stress, and promotes apoptosis. Fusion of
this gene and FUS on chromosome 16 or EWSR1 on chromosome 22
induced by translocation generates chimeric proteins in myxoid
liposarcomas or Ewing sarcoma. Multiple alternatively spliced
transcript variants encoding two isoforms with different length
have been identified.
Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010)
Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010)
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Wang, Y.L., et al. J. Exp. Clin. Cancer Res. 29, 54 (2010) :
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Uniprot Description
CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
Protein type: Transcription factor; Oncoprotein; DNA-binding; Autophagy
Chromosomal Location of Human Ortholog: 12q13.1-q13.2
Cellular Component: nucleoplasm; late endosome; cytosol; nucleus
Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription corepressor activity; transcription factor activity
Biological Process: regulation of transcription in response to stress; proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; unfolded protein response; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; positive regulation of interleukin-8 production; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; cell redox homeostasis; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of myoblast differentiation; cell cycle arrest; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; response to DNA damage stimulus; ER overload response
Disease: Myxoid Liposarcoma
Research Articles on DDIT3
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Product Notes
The DDIT3 ddit3 (Catalog #AAA9209135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 120-149. The DDIT3 Antibody (C-term A135) reacts with Mouse (Predicted Reactivity: Bovine, Hamster) and may cross-react with other species as described in the data sheet. AAA Biotech's DDIT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the DDIT3 ddit3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDIT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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