Human apolipoprotein L, 1 ELISA Kit | APOL1 elisa kit

Human Apolipoprotein L1, APOL1 ELISA Kit

Cat. Num. AAA920884

• Gene Names: APOL1; APOL; APO-L; FSGS4; APOL-I
• Reactivity: Human
• Synonyms: apolipoprotein L; 1; Human Apolipoprotein L1; APOL1 ELISA Kit; Human Apolipoprotein L1 (APOL1) ELISA kit; RP1-68O2.2; APO-L; APOL; APOL-I; OTTHUMP00000197984; apolipoprotein L-I; apolipoprotein L1; APOL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human APOL1. No significant cross-reactivity or interference between Human APOL1 and analogues was observed.
• Sequence Length: 398

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 7.81 ng/ml-500 ng/ml
• Sensitivity: 1.95 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 2: Lipogenesis and lipometabolism
• Protein Biological Process 3: Cholesterol metabolism
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOL1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with APOL1. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for APOL1C mix well and incubated. The more the amount of APOL1 in samples, the less HRP-antibody bound by pre-coated APOL1. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of APOL1 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 211938440
• NCBI GeneID: 8542
• NCBI Accession #: NP_001130012.1
• NCBI GenBank Nucleotide #: NM_001136540.1
• UniProt Accession #: O14791; O60804; Q5R3P7; Q5R3P8; Q96AB8; Q96PM4; Q9BQ03; A5PLQ4; B4DU12; E9PF24
• Molecular Weight: 43,974 Da
• NCBI Official Full Name: apolipoprotein L1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein L, 1
• NCBI Official Symbol: APOL1
• NCBI Official Synonym Symbols: APOL; APO-L; FSGS4; APOL-I
• NCBI Protein Information: apolipoprotein L1
• UniProt Protein Name: Apolipoprotein L1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOL1
• UniProt Synonym Gene Names: APOL; Apo-L; ApoL; ApoL-I
• UniProt Entry Name: APOL1_HUMAN

NCBI Description

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Lipid-binding; Secreted; Channel, chloride

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; extracellular region; intrinsic to membrane

Molecular Function: chloride channel activity; protein binding; lipid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; killing of cells of another organism; cytolysis; innate immune response; chloride transport; lipoprotein metabolic process; lipid transport

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To