Rabbit anti-Human USP19 Polyclonal Antibody | anti-USP19 antibody

USP19 Antibody (C-term)

Cat. Num. AAA9207748

• Gene Names: USP19; ZMYND9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: USP19; Polyclonal Antibody; USP19 Antibody (C-term); Ubiquitin carboxyl-terminal hydrolase 19; Deubiquitinating enzyme 19; Ubiquitin thioesterase 19; Ubiquitin-specific-processing protease 19; Zinc finger MYND domain-containing protein 9; KIAA0891; ZMYND9; anti-USP19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This USP19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1289-1319 amino acids from the C-terminal region of human USP19.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1289-1319
• Sequence Length: 1419

• Applicable Applications for anti-USP19 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of hUSP19-L1304 in Jurkat cell line lysates (35ug/lane). USP19 (arrow) was detected using the purified Pab.)

Related Product Information for anti-USP19 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP19 antibody

Cell Biology

References

Nagase, T., et al., DNA Res. 5(6):355-364 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 312596871
• NCBI GeneID: 10869
• NCBI Accession #: NP_001186089.1; NP_001186090.1; NP_001186091.1; NP_006668.1
• NCBI GenBank Nucleotide #: NM_001199160.1
• UniProt Accession #: O94966; Q3KQW4; Q641Q9; Q6NZY8; Q86XV9; A5PKX8; A6H8U2; B4DGT3; B4DTZ0; E7EN22; E7ETS0; E9PEG8
• Molecular Weight: 145651
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 19 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 19
• NCBI Official Symbol: USP19
• NCBI Official Synonym Symbols: ZMYND9
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 19
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 19
• UniProt Gene Name: USP19
• UniProt Synonym Gene Names: KIAA0891; ZMYND9
• UniProt Entry Name: UBP19_HUMAN

Uniprot Description

USP19: Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin- dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic- reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non- catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains. Belongs to the peptidase C19 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Ubiquitin conjugating system; EC 3.4.19.12; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; integral to membrane; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; metal ion binding; ubiquitin-specific protease activity

Biological Process: negative regulation of skeletal muscle development; ER-associated protein catabolic process; protein deubiquitination; regulation of protein stability