Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (All lanes : Anti-RPL28 Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human RPL28 Polyclonal Antibody | anti-RPL28 antibody

RPL28 Antibody

Gene Names
RPL28; L28
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RPL28; Polyclonal Antibody; RPL28 Antibody; 60S ribosomal protein L28; anti-RPL28 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
21-80
Sequence Length
87
Applicable Applications for anti-RPL28 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human RPL28
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-RPL28 Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RPL28 Antibody at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
References
Frigerio J.-M.,et al.Biochim. Biophys. Acta 1262:64-68(1995).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15748
NCBI Official Full Name
60S ribosomal protein L28 isoform 2
NCBI Official Synonym Full Names
ribosomal protein L28
NCBI Official Symbol
RPL28
NCBI Official Synonym Symbols
L28
NCBI Protein Information
60S ribosomal protein L28
UniProt Protein Name
60S ribosomal protein L28
Protein Family
UniProt Gene Name
RPL28
UniProt Entry Name
RL28_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Uniprot Description

RPL28: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: membrane; cytoplasm; dendrite; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; translational termination; viral infectious cycle

Research Articles on RPL28

Similar Products

Product Notes

The RPL28 rpl28 (Catalog #AAA9207328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-80. The RPL28 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPL28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin. WB~~ 1:1000. Researchers should empirically determine the suitability of the RPL28 rpl28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.