Rabbit anti-Human CA1 Polyclonal Antibody | anti-CA1 antibody

CA1 Antibody (N-term)

Cat. Num. AAA9207299

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CA1; Polyclonal Antibody; CA1 Antibody (N-term); Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I; anti-CA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human CA1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 60-90
• Sequence Length: 261

• Applicable Applications for anti-CA1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CA1 (arrow) using rabbit polyclonal CA1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA1 gene.)

Immunohistochemistry (IHC)

(CA1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CA1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CA1 antibody

Carbonic anhydrases (CAs) are a large family of zinc
metalloenzymes that catalyze the reversible hydration of carbon
dioxide. They participate in a variety of biological processes,
including respiration, calcification, acid-base balance, bone
resorption, and the formation of aqueous humor, cerebrospinal
fluid, saliva, and gastric acid. They show extensive diversity in
tissue distribution and in their subcellular localization. CA1 is
closely linked to CA2 and CA3 genes on chromosome 8, and it encodes
a cytosolic protein which is found at the highest level in
erythrocytes. Variants of this gene have been described in some
populations. Multiple alternatively spliced variants, encoding the
same protein, have been identified. Transcript variants of CA1
utilizing alternative polyA_sites have been described in
literature.

Product Categories/Family for anti-CA1 antibody

Cardiovascular; Cell biology

References

Abhary, S., et al. Mol. Vis. 15, 1179-1184 (2009) :
Gambhir, K.K., et al. Biochem. Genet. 45 (5-6), 431-439 (2007) :
Temperini, C., et al. Bioorg. Med. Chem. Lett. 17(8):2210-2215(2007)
Temperini, C., et al. Bioorg. Med. Chem. Lett. 16(19):5152-5156(2006)
Dawson, S.J., et al. J. Infect. 24(3):317-320(1992)

NCBI and Uniprot Product Information

• NCBI GI #: 192447430
• NCBI GeneID: 759
• NCBI Accession #: NP_001122301.1; NP_001122302.1; NP_001122303.1; NP_001158302.1; NP_001729.1
• NCBI GenBank Nucleotide #: NM_001128829.3
• UniProt Accession #: P00915
• Molecular Weight: 28870
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process