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Western Blot (WB) (SOHLH1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SOHLH1 antibody detected the SOHLH1 protein (arrow).)

Rabbit anti-Human SOHLH1 Polyclonal Antibody | anti-SOHLH1 antibody

SOHLH1 Antibody (N-term)

Gene Names
SOHLH1; TEB2; NOHLH; SPATA27; bHLHe80; C9orf157; bA100C15.3
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SOHLH1; Polyclonal Antibody; SOHLH1 Antibody (N-term); Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1; C9orf157; NOHLH; TEB2; anti-SOHLH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SOHLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-68 amino acids from the N-terminal region of human SOHLH1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
40-68
Sequence Length
387
Applicable Applications for anti-SOHLH1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(SOHLH1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SOHLH1 antibody detected the SOHLH1 protein (arrow).)

Western Blot (WB) (SOHLH1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SOHLH1 antibody detected the SOHLH1 protein (arrow).)

Immunohistochemistry (IHC)

(SOHLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOHLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (SOHLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOHLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SOHLH1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (SOHLH1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-SOHLH1 antibody
Probable transcription factor required during spermatogenesis and oogenesis (By similarity).
Product Categories/Family for anti-SOHLH1 antibody
References
Choi, Y., et al. Hum. Mutat. 31(7):788-793(2010)
Pangas, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 103(21):8090-8095(2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34526
NCBI Official Full Name
spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 isoform b
NCBI Official Synonym Full Names
spermatogenesis and oogenesis specific basic helix-loop-helix 1
NCBI Official Symbol
SOHLH1
NCBI Official Synonym Symbols
TEB2; NOHLH; SPATA27; bHLHe80; C9orf157; bA100C15.3
NCBI Protein Information
spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
UniProt Protein Name
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
UniProt Gene Name
SOHLH1
UniProt Synonym Gene Names
C9orf157; NOHLH; TEB2
UniProt Entry Name
SOLH1_HUMAN

NCBI Description

This gene encodes one of testis-specific transcription factors which are essential for spermatogenesis, oogenesis and folliculogenesis. This gene is located on chromosome 9. Mutations in this gene are associated with nonobstructive azoospermia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

SOHLH1: Transcription factor expressed in undifferentiated spermatogonia required for spermatogonial development. Genetic variations in SOHLH1 may be associated with non-obstructive azoospermia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription factor activity

Biological Process: oogenesis; ovarian follicle development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; spermatogenesis

Research Articles on SOHLH1

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Product Notes

The SOHLH1 sohlh1 (Catalog #AAA9207236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 40-68. The SOHLH1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOHLH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the SOHLH1 sohlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOHLH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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