Western Blot (WB)
(FAM105B Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the FAM105B antibody detected FAM105B protein (arrow).)
Rabbit anti-Human, mouse FAM105B Polyclonal Antibody | anti-OTULIN antibody
FAM105B Antibody (Center)
Gene Names
OTULIN; GUM; FAM105B
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FAM105B; Polyclonal Antibody; FAM105B Antibody (Center); Ubiquitin thioesterase otulin; Deubiquitinating enzyme otulin; OTU domain-containing deubiquitinase with linear linkage specificity; Ubiquitin thioesterase Gumby; OTULIN; anti-OTULIN antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FAM105B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-128 amino acids from the Central region of human FAM105B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
99-128
Sequence Length
352
Applicable Applications for anti-OTULIN antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(FAM105B Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the FAM105B antibody detected FAM105B protein (arrow).)
Western Blot (WB)
(FAM105B Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the FAM105B antibody detected FAM105B protein (arrow).)
Immunohistochemistry (IHC)
(FAM105B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FAM105B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FAM105B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FAM105B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(FAM105B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FAM105B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-OTULIN antibody
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40263
NCBI Official Full Name
ubiquitin thioesterase otulin
NCBI Official Synonym Full Names
OTU deubiquitinase with linear linkage specificity
NCBI Official Symbol
OTULIN
NCBI Official Synonym Symbols
GUM; FAM105B
NCBI Protein Information
ubiquitin thioesterase otulin
UniProt Protein Name
Ubiquitin thioesterase otulin
Protein Family
UniProt Gene Name
OTULIN
UniProt Synonym Gene Names
FAM105B
UniProt Entry Name
OTUL_HUMAN
Uniprot Description
FAM105B: Belongs to the FAM105 family.
Protein type: EC 3.4.19.12
Chromosomal Location of Human Ortholog: 5p15.2
Cellular Component: cytoplasm
Molecular Function: protein binding; ubiquitin-specific protease activity; cysteine-type peptidase activity
Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; innate immune response; Wnt receptor signaling pathway through beta-catenin; sprouting angiogenesis; proteolysis
Research Articles on OTULIN
Similar Products
Product Notes
The OTULIN otulin (Catalog #AAA9206835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 99-128. The FAM105B Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAM105B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the OTULIN otulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM105B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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