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Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Rabbit anti-Human AGAP8 Polyclonal Antibody | anti-AGAP8 antibody
AGAP8 Antibody (C-term)
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AGAP8; Polyclonal Antibody; AGAP8 Antibody (C-term); Arf-GAP with GTPase; ANK repeat and PH domain-containing protein 8; AGAP-8; Centaurin-gamma-like family member 5; CTGLF5; anti-AGAP8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AGAP8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 498-531 amino acids from the C-terminal region of human AGAP8.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
663
Applicable Applications for anti-AGAP8 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
FC~~1:25
IF~~1:25
IHC~~1:25
WB~~1:1000
IF~~1:25
IHC~~1:25
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Immunofluorescence (IF)
(Fluorescent image of HeLa cells stained with AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of HeLa cells stained with AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot analysis of lysate from HT-1080 cell line, using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from HT-1080 cell line, using AGAP8 Antibody (C-term). MBS9206649 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-AGAP8 antibody
Putative GTPase-activating protein (Potential).
Product Categories/Family for anti-AGAP8 antibody
References
Deloukas P.,et al.Nature 429:375-381(2004).
Varela I.,et al.Nature 469:539-542(2011).
Varela I.,et al.Nature 469:539-542(2011).
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
73068
NCBI Official Full Name
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 8
UniProt Protein Name
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 8
UniProt Gene Name
AGAP8
UniProt Synonym Gene Names
CTGLF5; AGAP-8
UniProt Entry Name
AGAP8_HUMAN
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Product Notes
The AGAP8 agap8 (Catalog #AAA9206649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGAP8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGAP8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC). FC~~1:25 IF~~1:25 IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the AGAP8 agap8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGAP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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