Rabbit Pyruvate Kinase (PKM2) Polyclonal Antibody | anti-PKM antibody

Pyruvate Kinase (PKM2) Antibody (N-term)

Cat. Num. AAA9205315

• Gene Names: PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human, mouse (Predicted Reactivity: Rabbit, Rat)
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Pyruvate Kinase (PKM2); Polyclonal Antibody; Pyruvate Kinase (PKM2) Antibody (N-term); Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP3; PK2; PK3; PKM2; anti-PKM antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Rabbit, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-151 amino acids from the N-terminal region of human Pyruvate Kinase (PKM2).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 121-151
• Sequence Length: 605

• Applicable Applications for anti-PKM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PKM2(K136) antibody at 1:2000 dilutionLane 1: MCF-7 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLane 4: PC-12 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-PKM2(K136) antibody at 1:2000 dilutionLane 1: MCF-7 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: THP-1 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLane 5: PC-12 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PKM antibody

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Product Categories/Family for anti-PKM antibody

Cancer; Cardiovascular; Metabolism; Signal transduction

References

Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).
Gress, T.M., et al., Oncogene 13(8):1819-1830 (1996).
Takenaka, M., et al., Eur. J. Biochem. 198(1):101-106 (1991).
Kato, H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(20):7861-7865 (1989).
Tsutsumi, H., et al., Genomics 2(1):86-89 (1988).

NCBI and Uniprot Product Information

• NCBI GI #: 332164775
• NCBI GeneID: 5315
• NCBI Accession #: NP_001193725.1; NP_001193726.1; NP_001193727.1; NP_001193728.1; NP_002645.3; NP_872270.1; NP_872271.1
• NCBI GenBank Nucleotide #: NM_001206796.1
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
• Molecular Weight: 57937
• NCBI Official Full Name: pyruvate kinase PKM isoform c
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: Nucleotide Metabolism - purine; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus; vesicle; cilium

Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation