Immunofluorescence (IF)
(Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term). MBS9204137 was diluted at 1?100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Rabbit anti-Human ALOX12 Polyclonal Antibody | anti-ALOX12 antibody
ALOX12 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term). MBS9204137 was diluted at 1?100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term). MBS9204137 was diluted at 1?100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Western Blot (WB)
(Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ALOX12 Antibody (C-term) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ALOX12 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.31; Apoptosis; Oxidoreductase; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: membrane; cytoplasm; cytosol; sarcolemma
Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; linoleic acid metabolic process; positive regulation of caspase activity; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; positive regulation of mitochondrial depolarization; lipoxygenase pathway; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; hepoxilin metabolic process; superoxide release; positive regulation of cell migration; aging; negative regulation of apoptosis
Research Articles on ALOX12
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Product Notes
The ALOX12 alox12 (Catalog #AAA9204137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 618-650. The ALOX12 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX12 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). IF~~1:100 WB~~1:1000. Researchers should empirically determine the suitability of the ALOX12 alox12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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