Western Blot (WB)
(All lanes : Anti-NFIC Antibody (Center) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human NFIC Polyclonal Antibody | anti-NFIC antibody
NFIC Antibody (Center)
Western Blot (WB)
(All lanes : Anti-NFIC Antibody (Center) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-NFIC Antibody (Center) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.)
Nilsson, J., et al. Cancer Res. 70(5):2020-2029(2010)
Udelhoven, M., et al. J. Mol. Endocrinol. 44(2):99-113(2010)
Plasari, G., et al. Mol. Cell. Biol. 29(22):6006-6017(2009)
Riffel, A.K., et al. Mol. Pharmacol. 76(5):1104-1114(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
NFI-C: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: double-stranded DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; odontogenesis of dentine-containing teeth
Research Articles on NFIC
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Product Notes
The NFIC nfic (Catalog #AAA9203883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 180-208. The NFIC Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFIC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the NFIC nfic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFIC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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