Rabbit RCL1 Polyclonal Antibody | anti-RCL1 antibody

RCL1 Antibody (N-term)

Cat. Num. AAA9203406

• Gene Names: RCL1; RNAC; RPCL1
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RCL1; Polyclonal Antibody; RCL1 Antibody (N-term); RNA 3'-terminal phosphate cyclase-like protein; RNAC; RPC2; RPCL1; RTC2; anti-RCL1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human RCL1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 14-43
• Sequence Length: 187

• Applicable Applications for anti-RCL1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-RCL1 Pab in A375 cell line lysate (35ug/lane). RCL1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RCL1 (arrow) using rabbit polyclonal RCL1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCL1 gene.)

Related Product Information for anti-RCL1 antibody

RCL1, which does not have cyclase activity, plays a role in 40S- ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA.

References

Billy, E., et al., EMBO J. 19(9):2115-2126 (2000).
Genschik P., et al., EMBO J. 16(10):2955-67 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 157426877
• NCBI GeneID: 10171
• NCBI Accession #: NP_005763.3
• NCBI GenBank Nucleotide #: NM_005772.4
• UniProt Accession #: Q9Y2P8; Q5VYW9; Q5VZU1; Q9H9D0; Q9NY00; Q9P044; D3DRI2
• Molecular Weight: 40843
• NCBI Official Full Name: RNA 3'-terminal phosphate cyclase-like protein isoform a
• NCBI Official Synonym Full Names: RNA terminal phosphate cyclase-like 1
• NCBI Official Symbol: RCL1
• NCBI Official Synonym Symbols: RNAC; RPCL1
• NCBI Protein Information: RNA 3'-terminal phosphate cyclase-like protein
• UniProt Protein Name: RNA 3'-terminal phosphate cyclase-like protein
• Protein Family: RNA 3'-terminal phosphate cyclase-like protein
• UniProt Gene Name: RCL1
• UniProt Synonym Gene Names: RNAC; RPC2; RPCL1; RTC2
• UniProt Entry Name: RCL1_HUMAN

Uniprot Description

RCL1: Does not have cyclase activity. Plays a role in 40S- ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 9p24.1-p23

Cellular Component: nucleolus

Molecular Function: endoribonuclease activity; RNA binding; RNA-3'-phosphate cyclase activity

Biological Process: RNA processing; endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)