Western Blot (WB)
(Western blot analysis of Kalirin expression in HEK293T (A), NIH3T3 (B), H9C2 (C), mouse brain (D) whole cell lysates.)
Kalirin Polyclonal Antibody | anti-KALRN antibody
Anti-Kalirin Antibody
IF/ICC: (1/100 - 1/500)
Western Blot (WB)
(Western blot analysis of Kalirin expression in HEK293T (A), NIH3T3 (B), H9C2 (C), mouse brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Kalirin expression in HEK293T (A), NIH3T3 (B), H9C2 (C), mouse brain (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Kalirin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Kalirin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
kalirin: a bifunctional enzyme with protein kinase activity and guanine-exchange factor (GEF) activity. A protein kinase of the CAMK group. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Genetic variants are associated with susceptibility to coronary heart disease type 5 (CHDS5), a multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1) Interacts with FASLG. The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. 6 isoforms of the human protein are produced by alternative splicing. Isoform 2 is brain specific and is highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
Protein type: Kinase, protein; GEFs, Rac/Rho; Protein kinase, CAMK; GEFs; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; Trio family
Chromosomal Location of Human Ortholog: 3q21.2
Cellular Component: cytosol; actin cytoskeleton
Molecular Function: protein serine/threonine kinase activity; Rho guanyl-nucleotide exchange factor activity; protein binding; metal ion binding; guanyl-nucleotide exchange factor activity; ATP binding
Biological Process: vesicle-mediated transport; axon guidance; nervous system development; regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; ephrin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of GTPase activity
Disease: Coronary Heart Disease, Susceptibility To, 5
Research Articles on KALRN
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Product Notes
The KALRN kalrn (Catalog #AAA821543) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kalirin Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Kalirin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC). WB: (1/500 - 1/1000) IF/ICC: (1/100 - 1/500). Researchers should empirically determine the suitability of the KALRN kalrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kalirin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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