Western Blot (WB)
(Western blot analysis of DDX23 Antibody (Center) in 293 cell line lysates (35ug/lane). DDX23 (arrow) was detected using the purified Pab.)
Rabbit anti-Human DDX23 Polyclonal Antibody | anti-DDX23 antibody
DDX23 Antibody (Center)
Western Blot (WB)
(Western blot analysis of DDX23 Antibody (Center) in 293 cell line lysates (35ug/lane). DDX23 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DDX23 Antibody (Center) in 293 cell line lysates (35ug/lane). DDX23 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(DDX23 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DDX23 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DDX23 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DDX23 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
DDX23: Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
Protein type: RNA processing; RNA splicing; Spliceosome; Helicase; RNA-binding; EC 3.6.4.13
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: nucleoplasm; mitochondrion; snRNP U5; nucleolus; plasma membrane; nucleus
Molecular Function: protein binding; ATP-dependent helicase activity; ATP-dependent RNA helicase activity; ATP binding
Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; cis assembly of pre-catalytic spliceosome; RNA splicing; gene expression
Research Articles on DDX23
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Product Notes
The DDX23 ddx23 (Catalog #AAA9203398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 248-277. The DDX23 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX23 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the DDX23 ddx23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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