Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Rabbit anti-Human SPT13 Polyclonal Antibody | anti-SPATA13 antibody
SPT13 Antibody (Center)
Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Western Blot (WB)
(SPT13 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SPT13 antibody detected the SPT13 protein (arrow).)
Immunohistochemistry (IHC)
(SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SPT13 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPT13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SPT13 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Bristow, J.M., et al. J. Cell. Sci. 122 (PT 24), 4535-4546 (2009)
Sagara, M., et al. Oncogene 28(10):1357-1365(2009)
NCBI and Uniprot Product Information
Uniprot Description
SPATA13 iso6: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up- regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. Interacts (via ABR and SH3 domain) with APC. The binding of APC enhances its GEF activity by relieving it from an autoinhibitory conformation, in which the ABR and SH3 domains are associated with the C-terminal tail. Interacts (via C-terminal tail) with PPP1R9B (via C-terminus). Interacts with RAC1. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs, Rac/Rho; GEFs
Chromosomal Location of Human Ortholog: 13q12.12
Cellular Component: nucleoplasm; lamellipodium; cytoplasm; filopodium
Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity
Biological Process: lamellipodium biogenesis; cell migration; filopodium formation; regulation of cell migration; positive regulation of GTPase activity
Research Articles on SPATA13
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Product Notes
The SPATA13 spata13 (Catalog #AAA9203062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 204-232. The SPT13 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPT13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the SPATA13 spata13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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