Rabbit IRAK4 Polyclonal Antibody | anti-IRAK4 antibody

IRAK4 Antibody (N-term)

Cat. Num. AAA9202564

• Gene Names: IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: IRAK4; Polyclonal Antibody; IRAK4 Antibody (N-term); Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64; anti-IRAK4 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This IRAK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-36 amino acids from the N-terminal region of human IRAK4.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 5-36
• Sequence Length: 336

• Applicable Applications for anti-IRAK4 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-IRAK4 Pab in Jurkat cell lysate. IRAK4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of IRAK4 (arrow) using rabbit polyclonal IRAK4 Antibody (R20). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRAK4 gene.)

Related Product Information for anti-IRAK4 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

Product Categories/Family for anti-IRAK4 antibody

Cardiovascular; Immunology; Signal Transduction

References

Medvedev, A.E., et al., J. Exp. Med. 198(4):521-531 (2003).
Jiang, Z., et al., J. Biol. Chem. 278(13):10952-10956 (2003).
Picard, C., et al., Science 299(5615):2076-2079 (2003).
Li, S., et al., Proc. Natl. Acad. Sci. U.S.A. 99(8):5567-5572 (2002).
Suzuki, N., et al., Nature 416(6882):750-756 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 166795295
• NCBI GeneID: 51135
• NCBI Accession #: NP_001107654.1; NP_001138728.1; NP_001138729.1; NP_001138730.1; NP_057207.2
• NCBI GenBank Nucleotide #: NM_001114182.2
• UniProt Accession #: Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589
• Molecular Weight: 51530
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 4 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; IRAK-4; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4
• UniProt Entry Name: IRAK4_HUMAN

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; cytokine production; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency