Western Blot (WB)
(Western blot analysis of anti-PRKAR1B Pab in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, mouse Protein Kinase A regulatory subunit I beta Polyclonal Antibody | anti-PRKAR1B antibody
Protein Kinase A regulatory subunit I beta Antibody (N-term)
Western Blot (WB)
(Western blot analysis of anti-PRKAR1B Pab in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-PRKAR1B Pab in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
responses to external signals such as proliferation, ion transport, regulation
of metabolism and gene transcription by activation of the cAMP-dependent
protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to
the two regulatory subunits of the tetrameric PKA holoenzyme resulting in
release of active catalytic subunits. Three catalytic (C) subunits have been
identified, designated Cα, Cβ and Cγ, that each represent specific gene
products. Cα and Cβ are closely related (93% amino acid sequence similari-
ty), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively.
Activation of transcription upon elevation of cAMP levels results from
translocation of PKA to the nucleus where it phosphorylates the transcrip-
tion factor cAMP response element binding protein (CREB) on serine 133
which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus
linking phospho-CREB to the pol II transcription initiation complex.
Solberg, R., et al., Biochem. Biophys. Res. Commun. 176(1):166-172 (1991).
NCBI and Uniprot Product Information
NCBI Description
Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009]
Uniprot Description
PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family.
Protein type: Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 7p22
Cellular Component: plasma membrane; cytosol; cAMP-dependent protein kinase complex
Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; protein amino acid phosphorylation; learning and/or memory; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport
Research Articles on PRKAR1B
Similar Products
Product Notes
The PRKAR1B prkar1b (Catalog #AAA9202454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 50-80. The Protein Kinase A regulatory subunit I beta Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase A regulatory subunit I beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PRKAR1B prkar1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein Kinase A regulatory subunit I beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
