Western Blot (WB)
(The anti-NPK Pab is used in Western blot to detect NPK in Jurkat cell lysate.)
Rabbit anti-Human TRIB3 Polyclonal Antibody | anti-TRIB3 antibody
TRIB3 Antibody (N-term)
Western Blot (WB)
(The anti-NPK Pab is used in Western blot to detect NPK in Jurkat cell lysate.)
Western Blot (WB)
(The anti-NPK Pab is used in Western blot to detect NPK in Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of NPK(arrow) using rabbit polyclonal NPK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB3 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of NPK(arrow) using rabbit polyclonal NPK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB3 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Du, K., et al., Science 300(5625):1574-1577 (2003).
Bowers, A.J., et al., Oncogene 22(18):2823-2835 (2003).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.
Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family
Chromosomal Location of Human Ortholog: 20p13-p12.2
Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; protein kinase inhibitor activity; ubiquitin-protein ligase regulator activity; ubiquitin protein ligase binding; kinase activity; protein kinase binding; transcription corepressor activity; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; positive regulation of protein binding; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of ubiquitin-protein ligase activity; insulin receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process
Research Articles on TRIB3
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Product Notes
The TRIB3 trib3 (Catalog #AAA9201591) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 15-45. The TRIB3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TRIB3 trib3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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