Western Blot (WB)
(All lanes : Anti-CHAC1 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: PC-12 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit CHAC1 Polyclonal Antibody | anti-CHAC1 antibody
CHAC1 Antibody
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHAC1; Polyclonal Antibody; CHAC1 Antibody; Glutathione-specific gamma-glutamylcyclotransferase 1; Gamma-GCG 1; 232-; Blocks Notch protein; Botch; Cation transport regulator-like protein 1; CHAC1 {ECO:0000303|PubMed:19109178; ECO:0000312|HGNC:HGNC:28680}; anti-CHAC1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
11-70
Sequence Length
219
Applicable Applications for anti-CHAC1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human CHAC1
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt
Western Blot (WB)
(All lanes : Anti-CHAC1 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: PC-12 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-CHAC1 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: PC-12 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CHAC1 antibody
Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. May also act as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade.
Product Categories/Family for anti-CHAC1 antibody
References
Zody M.C.,et al.Nature 440:671-675(2006).
Zheng J.M.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Mungrue I.N.,et al.J. Immunol. 182:466-476(2009).
Chi Z.,et al.Dev. Cell 22:707-720(2012).
Zheng J.M.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Mungrue I.N.,et al.J. Immunol. 182:466-476(2009).
Chi Z.,et al.Dev. Cell 22:707-720(2012).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28747
NCBI Official Full Name
glutathione-specific gamma-glutamylcyclotransferase 1 isoform b
NCBI Official Synonym Full Names
ChaC glutathione-specific gamma-glutamylcyclotransferase 1
NCBI Official Symbol
CHAC1
NCBI Protein Information
glutathione-specific gamma-glutamylcyclotransferase 1
UniProt Protein Name
Glutathione-specific gamma-glutamylcyclotransferase 1
Protein Family
UniProt Gene Name
CHAC1
UniProt Synonym Gene Names
Botch
UniProt Entry Name
CHAC1_HUMAN
Uniprot Description
CHAC1: Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. May also act as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Belongs to the chaC family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: trans-Golgi network; cytosol
Molecular Function: protein binding; transferase activity, transferring acyl groups; Notch binding
Biological Process: Notch signaling pathway; neurogenesis; metabolic process; negative regulation of Notch signaling pathway; response to unfolded protein
Research Articles on CHAC1
Similar Products
Product Notes
The CHAC1 chac1 (Catalog #AAA9201481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 11-70. The CHAC1 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:1000. Researchers should empirically determine the suitability of the CHAC1 chac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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