Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Ki67 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180.)
Rabbit Ki67 Polyclonal Antibody | anti-Ki67 antibody
Ki67 Rabbit pAb
IHC: 1:50-1:200
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Ki67 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Ki67 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon using Ki67 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon using Ki67 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat spleen using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat spleen using Ki67 antibody at dilution of 1:50 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Ki67 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Ki67 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Uniprot Description
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
Protein type: Nucleolus; Cell cycle regulation
Chromosomal Location of Human Ortholog: 10q26.2
Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein binding; ATP binding
Biological Process: cell proliferation; meiosis
Research Articles on Ki67
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Product Notes
The Ki67 mki67 (Catalog #AAA9142524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ki67 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ki67 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the Ki67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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