Chicken MPO ELISA Kit | MPO elisa kit

Chicken MPO ELISA Kit

Cat. Num. AAA9141210

• Reactivity: Chicken
• Synonyms: MPO; Chicken MPO ELISA Kit; Chicken Myeloperoxidase(MPO); MPO elisa kit

• Reactivity: Chicken
• Sequence Length: 745

• Assay Type: Competitive ELISA
• Samples: Cell Culture Supernatant, Urine, Serum, Plasma
• Conjugate: HRP
• Detection Principle: Colorimetric
• Preparation and Storage: Store at 4 degree C.

Related Product Information for MPO elisa kit

Intended Use: This Chicken MPO ELISA kit is intended for laboratory research use only. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken MPO in the sample, this Chicken MPO ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Chicken MPO concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).

Background: As an analytic biochemistry assay, ELISA involves detection of an 'analyte' (i.e. the specific substance whose presence is being quantitatively or qualitatively analyzed) in a liquid sample by a method that continues to use liquid reagents during the 'analysis' (i.e. controlled sequence of biochemical reactions that will generate a signal which can be easily quantified and interpreted as a measure of the amount of analyte in the sample) that stays liquid and remains inside a reaction chamber or well needed to keep the reactants contained.As a heterogenous assay, ELISA separates some component of the analytical reaction mixture by adsorbing certain components onto a solid phase which is physically immobilized. In ELISA, a liquid sample is added onto a stationary solid phase with special binding properties and is followed by multiple liquid reagents that are sequentially added, incubated and washed followed by some optical change (e.g. color development by the product of an enzymatic reaction) in the final liquid in the well from which the quantity of the analyte is measured. The qualitative 'reading' usually based on detection of intensity of transmitted light by spectrophotometry, which involves quantitation of transmission of some specific wavelength of light through the liquid (as well as the transparent bottom of the well in the multiple-well plate format). The sensitivity of detection depends on amplification of the signal during the analytic reactions. Since enzyme reactions are very well known amplification processes, the signal is generated by enzymes which are linked to the detection reagents in fixed proportions to allow accurate quantification-thus the name 'enzyme linked'.The analyte is also called the ligand because it will specifically bind or ligate to a detection reagent, thus ELISA falls under the bigger category of ligand binding assays. The ligand-specific binding reagent is 'immobilized', i.e., usually coated and dried onto the transparent bottom and sometimes also side wall of a well (the stationary 'solid phase 'solid substrate' here as opposed to solid microparticle/beads that can be washed away), which is usually constructed as a multiple-well plate known as the 'ELISA plate'. Conventionally, like other forms of immunoassays, the specificity of antigen-antibody type reaction is used because it is easy to raise an antibody specifically against an antigen in bulk as a reagent. Alternatively, if the analyte itself is an antibody, its target antigen can be used as the binding reagent.

NCBI and Uniprot Product Information

• NCBI GI #: 4557759
• NCBI GeneID: 4353
• NCBI GenBank Nucleotide #: NP_000241.1
• UniProt Accession #: P05164
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.11.2.2

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; response to lipopolysaccharide; response to yeast; defense response; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease