Mouse Annexin II ELISA Kit | ANN-II elisa kit

Mouse Annexin II ELISA Kit

Cat. Num. AAA9135

• Gene Names: ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV
• Reactivity: Mouse
• Synonyms: Annexin II; Mouse Annexin II ELISA Kit; ANN-II elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse ANN-II and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.5 ng/ml - 16 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ANN-II concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse ANN-II antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANN-II and ANN-II antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANN-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ANN-II elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ANN-II concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse ANN-II antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ANN-II and ANN-II antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANN-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 209862831
• NCBI GeneID: 302
• NCBI Accession #: NP_001129487.1
• NCBI GenBank Nucleotide #: NM_001136015.2
• UniProt Accession #: P07355; Q567R4; Q6N0B3; Q8TBV2; Q96DD5; Q9UDH8
• Molecular Weight: 38,604 Da
• NCBI Official Full Name: annexin A2 isoform 2
• NCBI Official Synonym Full Names: annexin A2
• NCBI Official Symbol: ANXA2
• NCBI Official Synonym Symbols: P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV
• NCBI Protein Information: annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV
• UniProt Protein Name: Annexin A2
• UniProt Gene Name: ANXA2
• UniProt Synonym Gene Names: ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV
• UniProt Entry Name: ANXA2_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: extrinsic to plasma membrane; extracellular space; protein complex; cell surface; late endosome membrane; lysosomal membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; basement membrane; midbody; nucleus; endosome; sarcolemma; vesicle

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding

Biological Process: fibrinolysis; positive regulation of fibroblast proliferation; collagen fibril organization; negative regulation of catalytic activity; positive regulation of vesicle fusion; protein heterotetramerization; positive regulation of protein amino acid phosphorylation; angiogenesis; positive regulation of binding; body fluid secretion; lipid raft formation; membrane budding