Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit anti-Human, Mouse TGFBRAP1 Polyclonal Antibody | anti-TGFBRAP1 antibody
TGFBRAP1 Polyclonal Antibody
Gene Names
TGFBRAP1; VPS3; TRAP1; TRAP-1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
TGFBRAP1; Polyclonal Antibody; TGFBRAP1 Polyclonal Antibody; TRAP-1; TRAP1; VPS3; anti-TGFBRAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
LLQRASASGKGAEATETQAKLRRLLQKSDLYRVHFLLERLQGAGLPMESAILHGKLGEHEKALHILVHELQDFAAAEDYCLWCSEGRDPPHRQQLFHTLLAIYLHAGPTAHELAVAAVDLLNRHATEFDAAQVLQMLPDTWSVQLLCPFLMGAMRDSIHARRTMQVALGLARSENLIYTYDKMKLKGSSIQLSDKKLCQICQNPFCEPVFVRYPNGGLVHTHCAASRHTNPSSSSPGTRT
Sequence Length
860
Applicable Applications for anti-TGFBRAP1 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human TGFBRAP1
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm, Early endosome
Positive Samples
Raji, HeLa, Mouse brain, Mouse kidney
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-TGFBRAP1 antibody
This gene encodes a protein that binds to transforming growth factor-beta (TGF-beta) receptors and plays a role in TGF-beta signaling. The encoded protein acts as a chaprone in signaling downstream of TGF-beta. It is involved in signal-dependent association with SMAD4. The protein is also a component of mammalian CORVET, a multisubunit tethering protein complex that is involved in fusion of early endosomes.
Product Categories/Family for anti-TGFBRAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 97kDa
Observed: 100kDa
Observed: 100kDa
NCBI Official Full Name
transforming growth factor-beta receptor-associated protein 1 isoform 1
NCBI Official Synonym Full Names
transforming growth factor beta receptor associated protein 1
NCBI Official Symbol
TGFBRAP1
NCBI Official Synonym Symbols
VPS3; TRAP1; TRAP-1
NCBI Protein Information
transforming growth factor-beta receptor-associated protein 1
UniProt Protein Name
Transforming growth factor-beta receptor-associated protein 1
Protein Family
UniProt Gene Name
TGFBRAP1
UniProt Synonym Gene Names
TGF-beta receptor-associated protein 1; TRAP-1; TRAP1
NCBI Description
This gene encodes a protein that binds to transforming growth factor-beta (TGF-beta) receptors and plays a role in TGF-beta signaling. The encoded protein acts as a chaprone in signaling downstream of TGF-beta. It is involved in signal-dependent association with SMAD4. The protein is also a component of mammalian CORVET, a multisubunit tethering protein complex that is involved in fusion of early endosomes. [provided by RefSeq, Jun 2016]
Uniprot Description
Plays a role in the TGF-beta/activin signaling pathway. It associates with inactive heteromeric TGF-beta and activin receptor complexes, mainly through the type II receptor, and is released upon activation of signaling. May recruit SMAD4 to the vicinity of the receptor complex and facilitate its interaction with receptor-regulated Smads, such as SMAD2.
Research Articles on TGFBRAP1
Similar Products
Product Notes
The TGFBRAP1 tgfbrap1 (Catalog #AAA9134391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGFBRAP1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TGFBRAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the TGFBRAP1 tgfbrap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LLQRASASGK GAEATETQAK LRRLLQKSDL YRVHFLLERL QGAGLPMESA ILHGKLGEHE KALHILVHEL QDFAAAEDYC LWCSEGRDPP HRQQLFHTLL AIYLHAGPTA HELAVAAVDL LNRHATEFDA AQVLQMLPDT WSVQLLCPFL MGAMRDSIHA RRTMQVALGL ARSENLIYTY DKMKLKGSSI QLSDKKLCQI CQNPFCEPVF VRYPNGGLVH THCAASRHTN PSSSSPGTRT. It is sometimes possible for the material contained within the vial of "TGFBRAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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