Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using BFSP2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit anti-Mouse BFSP2 Polyclonal Antibody | anti-BFSP2 antibody
BFSP2 Polyclonal Antibody
Gene Names
BFSP2; CP47; CP49; LIFL-L; CTRCT12; PHAKOSIN
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
BFSP2; Polyclonal Antibody; BFSP2 Polyclonal Antibody; CP47; CP49; CTRCT12; LIFL-L; PHAKOSIN; anti-BFSP2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
VEYMAKVHALEQVSQELETQLRMHLESKATRSGNWGALRASWASSCQQVGEAVLENARLMLQTETIQAGADDFKERYENEQPFRKAAEEEINSLYKVIDEANLTKMDLESQIESLKEELGSLSRNYEEDVKLLHKQLAGCELEQMDAPIGTGLDDILETIRIQWERDVEKNRVEAGALLQAKQQAEVAHMSQTQEEKLAAALRVELHNTSCQVQSLQAETESLRALKRGLENTLHDAKHWHDMELQNLGAVVGRL
Sequence Length
415
Applicable Applications for anti-BFSP2 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of human BFSP2
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cell cortex, cytoskeleton
Positive Samples
Mouse eye
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using BFSP2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse eye, using BFSP2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Related Product Information for anti-BFSP2 antibody
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex.
Product Categories/Family for anti-BFSP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 45kDa
Observed: 46kDa
Observed: 46kDa
NCBI Official Full Name
phakinin
NCBI Official Synonym Full Names
beaded filament structural protein 2
NCBI Official Symbol
BFSP2
NCBI Official Synonym Symbols
CP47; CP49; LIFL-L; CTRCT12; PHAKOSIN
NCBI Protein Information
phakinin
UniProt Protein Name
Phakinin
Protein Family
UniProt Gene Name
BFSP2
UniProt Synonym Gene Names
CP47; CP49; LIFL-L
NCBI Description
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex. [provided by RefSeq, Jun 2009]
Uniprot Description
Involved in stabilization of lens fiber cell cytoskeleton.
Research Articles on BFSP2
Similar Products
Product Notes
The BFSP2 bfsp2 (Catalog #AAA9133104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BFSP2 Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BFSP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the BFSP2 bfsp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VEYMAKVHAL EQVSQELETQ LRMHLESKAT RSGNWGALRA SWASSCQQVG EAVLENARLM LQTETIQAGA DDFKERYENE QPFRKAAEEE INSLYKVIDE ANLTKMDLES QIESLKEELG SLSRNYEEDV KLLHKQLAGC ELEQMDAPIG TGLDDILETI RIQWERDVEK NRVEAGALLQ AKQQAEVAHM SQTQEEKLAA ALRVELHNTS CQVQSLQAET ESLRALKRGL ENTLHDAKHW HDMELQNLGA VVGRL. It is sometimes possible for the material contained within the vial of "BFSP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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