Rabbit anti-Human BRAT1 Polyclonal Antibody | anti-BRAT1 antibody

BRAT1 Polyclonal Antibody

Cat. Num. AAA9132619

• Gene Names: BRAT1; BAAT1; RMFSL; C7orf27
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: BRAT1; Polyclonal Antibody; BRAT1 Polyclonal Antibody; BAAT1; C7orf27; RMFSL; anti-BRAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 821

• Applicable Applications for anti-BRAT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human BRAT1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using Brat1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Related Product Information for anti-BRAT1 antibody

The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM.

Product Categories/Family for anti-BRAT1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 134047724
• NCBI GeneID: 221927
• UniProt Accession #: Q6PJG6; Q8IW85; Q8IZ43; Q8WVR8; Q96IV9; Q9H7J8; Q9UFA3; A4D200; C9JY24
• Molecular Weight: 88kDa
• NCBI Official Full Name: BRCA1-associated ATM activator 1
• NCBI Official Synonym Full Names: BRCA1 associated ATM activator 1
• NCBI Official Symbol: BRAT1
• NCBI Official Synonym Symbols: BAAT1; RMFSL; C7orf27
• NCBI Protein Information: BRCA1-associated ATM activator 1
• UniProt Protein Name: BRCA1-associated ATM activator 1
• Protein Family: BRCA1-associated ATM activator
• UniProt Gene Name: BRAT1
• UniProt Synonym Gene Names: BAAT1; C7orf27

NCBI Description

The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012]

Uniprot Description

BRAT1: Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM. Defects in BRAT1 are the cause of rigidity and multifocal seizure syndrome, lethal neonatal (RMFSL). A lethal, neonatal, neurologic disorder characterized by episodic jerking that is apparent in utero, lack of psychomotor development, axial and limb rigidity, frequent multifocal seizures, and dysautonomia. At birth, affected individuals have small heads, overlapping cranial sutures, small or absent fontanels, and depressed frontal bones. Infants show poorly responsive focal jerks of the tongue, face and arms in a nearly continuous sequence throughout life. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: apoptosis; cell growth; cell migration; cell proliferation; glucose metabolic process; mitochondrion localization; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; response to ionizing radiation

Disease: Rigidity And Multifocal Seizure Syndrome, Lethal Neonatal