Rabbit ROCK1 Monoclonal Antibody | anti-ROCK1 antibody

ROCK1 Monoclonal Antibody

Cat. Num. AAA9131351

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: ROCK1 ; Monoclonal Antibody; ROCK1 Monoclonal Antibody ; P160ROCK; ROCK-I; anti-ROCK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1354

• Applicable Applications for anti-ROCK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200; IP: 1:20 - 1:50
• Immunogen: Recombinant protein of human ROCK1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ROCK1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse thyroid using ROCK1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid using ROCK1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart using ROCK1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-ROCK1 antibody

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.

Product Categories/Family for anti-ROCK1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 47605999
• NCBI GeneID: 6093
• UniProt Accession #: Q13464; Q2KHM4; Q59GZ4; B0YJ91
• Molecular Weight: 158kDa
• NCBI Official Full Name: Rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho associated coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1
• UniProt Protein Name: Rho-associated protein kinase 1
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK

NCBI Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: AGC group; DMPK family; EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); ROCK subfamily

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: cytoskeleton; cytosol; extracellular region; lamellipodium; plasma membrane; ruffle

Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: actin cytoskeleton organization and biogenesis; cortical actin cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; I-kappaB kinase/NF-kappaB cascade; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; membrane to membrane docking; myoblast migration; negative regulation of angiogenesis; negative regulation of protein binding; neutrophil degranulation; positive regulation of focal adhesion formation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell adhesion; regulation of focal adhesion formation; regulation of keratinocyte differentiation; regulation of stress fiber formation; signal transduction; smooth muscle contraction; vascular endothelial growth factor receptor signaling pathway