Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FUBP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse FUBP3 Polyclonal Antibody | anti-FUBP3 antibody
FUBP3 Polyclonal Antibody
Gene Names
FUBP3; FBP3
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
FUBP3; Polyclonal Antibody; FUBP3 Polyclonal Antibody; anti-FUBP3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
572
Applicable Applications for anti-FUBP3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human FUBP3
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FUBP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FUBP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using FUBP3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using FUBP3 antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62kDa
NCBI Official Full Name
far upstream element-binding protein 3
NCBI Official Synonym Full Names
far upstream element (FUSE) binding protein 3
NCBI Official Symbol
FUBP3
NCBI Official Synonym Symbols
FBP3
NCBI Protein Information
far upstream element-binding protein 3
UniProt Protein Name
Far upstream element-binding protein 3
Protein Family
UniProt Gene Name
FUBP3
UniProt Synonym Gene Names
FBP3; FUSE-binding protein 3
UniProt Entry Name
FUBP3_HUMAN
Uniprot Description
FBP3: a nucleotide binding protein that interacts with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Two alternatively spliced isoforms have been described.
Protein type: Transcription factor; RNA-binding
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: membrane; cytoplasm; nucleus
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Research Articles on FUBP3
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Product Notes
The FUBP3 fubp3 (Catalog #AAA9128771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FUBP3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FUBP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB 1:500 - 1:2000 IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the FUBP3 fubp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUBP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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