Rabbit anti-Human, Mouse ABCB11 Polyclonal Antibody | anti-ABCB11 antibody

ABCB11 Polyclonal Antibody

Cat. Num. AAA9128561

• Gene Names: ABCB11; BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ABCB11; Polyclonal Antibody; ABCB11 Polyclonal Antibody; anti-ABCB11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1,321

• Applicable Applications for anti-ABCB11 antibody: Western Blot (WB)
• Application Notes: WB 1:500 - 1:2000
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human ABCB11
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ABCB11 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)

Related Product Information for anti-ABCB11 antibody

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy.

NCBI and Uniprot Product Information

• NCBI GI #: 21536378
• NCBI GeneID: 8647
• NCBI Accession #: NP_003733.2
• NCBI GenBank Nucleotide #: NM_003742.2
• UniProt Accession #: O95342; Q53TL2; Q9UNB2
• Molecular Weight: 146kDa
• NCBI Official Full Name: bile salt export pump
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• NCBI Official Symbol: ABCB11
• NCBI Official Synonym Symbols: BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• NCBI Protein Information: bile salt export pump
• UniProt Protein Name: Bile salt export pump
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB11
• UniProt Synonym Gene Names: BSEP
• UniProt Entry Name: ABCBB_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Transporter; Membrane protein, integral; Hydrolase; Membrane protein, multi-pass; Transporter, ABC family

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; apical part of cell; plasma membrane; integral to membrane

Molecular Function: transporter activity; ATPase activity, coupled to transmembrane movement of substances; canalicular bile acid transmembrane transporter activity; bile acid-exporting ATPase activity; sodium-exporting ATPase activity, phosphorylative mechanism; ATP binding

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; transport; bile acid metabolic process; canalicular bile acid transport; transmembrane transport

Disease: Cholestasis, Progressive Familial Intrahepatic, 2; Cholestasis, Benign Recurrent Intrahepatic, 2