Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 antibody(MBS9127392) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 300s.)
Rabbit H4-R3 Antibody | anti-H4R3me2a antibody
Asymmetric DiMethyl-Histone H4-R3
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
H4-R3; Asymmetric DiMethyl-Histone H4-R3; FO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A; anti-H4R3me2a antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Applicable Applications for anti-H4R3me2a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Species
Human
Route
Synthetic Peptide
Immunogen
A synthetic peptide of human HIST2H4A
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 antibody(MBS9127392) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 300s.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 antibody(MBS9127392) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 300s.)
Dot Blot (DB)
(Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (MBS9127392) at 1:1000 dilution.)
Dot Blot (DB)
(Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (MBS9127392) at 1:1000 dilution.)
Related Product Information for anti-H4R3me2a antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replicationdependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Product Categories/Family for anti-H4R3me2a antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The H4R3me2a (Catalog #AAA9127392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Asymmetric DiMethyl-Histone H4-R3 reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. AAA Biotech's H4-R3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200. Researchers should empirically determine the suitability of the H4R3me2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H4-R3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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