Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human, Mouse Eif2ak2 Polyclonal Antibody | anti-Eif2ak2 antibody
Eif2ak2 Polyclonal Antibody
IHC: 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
Uniprot Description
PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.
Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.10.2; Translation; Other group; PEK family
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; RNA binding; protein-tyrosine kinase activity; double-stranded RNA binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity
Biological Process: positive regulation of cytokine production; translation; transcription, DNA-dependent; activation of MAPKK activity; positive regulation of apoptosis; immune system process; unfolded protein response; response to toxin; response to virus; protein amino acid autophosphorylation; response to lipopolysaccharide; protein amino acid phosphorylation; positive regulation of chemokine production; positive regulation of stress-activated MAPK cascade; activation of NF-kappaB transcription factor; response to vitamin E; negative regulation of viral genome replication; regulation of transcription, DNA-dependent; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; phosphorylation; defense response to virus; negative regulation of apoptosis
Research Articles on Eif2ak2
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Product Notes
The Eif2ak2 eif2ak2 (Catalog #AAA9125694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eif2ak2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Eif2ak2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the Eif2ak2 eif2ak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eif2ak2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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