Rabbit LILRB4 Antibody | anti-LILRB4 antibody

LILRB4

Cat. Num. AAA9125297

• Gene Names: LILRB4; ILT3; LIR5; CD85K; ILT-3; LIR-5
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: LILRB4; CD85K; ILT-3; ILT3; LIR-5; LIR5; anti-LILRB4 antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-LILRB4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1200; IHC: 1:50-1:200; IF: 1:50-1:200
• Species Reactivity/Cross-Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-259 of human LILRB4 (NP_001265355.2).
• Category: Primary Antibody
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LILRB4 antibody (MBS9125297) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using LILRB4 Rabbit pAb (MBS9125297) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using LILRB4 Rabbit pAb (MBS9125297) at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of mouse spleen using LILRB4 Rabbit pAb (MBS9125297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-LILRB4 antibody

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen- presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-LILRB4 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 322510117
• NCBI GeneID: 11006
• UniProt Accession #: Q8NHJ6; O15468; O75021; Q6FGQ9; Q8N1C7; Q8NHL5; A8MVL8
• Molecular Weight: Observed MW: 49KDa; Calculated MW: 49kDa
• NCBI Official Full Name: Leukocyte immunoglobulin-like receptor subfamily B member 4
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
• NCBI Official Symbol: LILRB4
• NCBI Official Synonym Symbols: ILT3; LIR5; CD85K; ILT-3; LIR-5
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 4; immunoglobulin-like transcript 3; CD85 antigen-like family member K; monocyte inhibitory receptor HM18; leukocyte immunoglobulin-like receptor 5
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 4
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB4
• UniProt Synonym Gene Names: ILT3; LIR5; ILT-3; LIR-5
• UniProt Entry Name: LIRB4_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB4: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5- signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; receptor activity; antigen binding

Biological Process: negative regulation of osteoclast differentiation; immune system process; signal transduction