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Typical Testing Data/Standard Curve (for reference only)

Human CD70 molecule ELISA Kit | CD70 elisa kit

Human CD70 antigen, CD70 ELISA Kit

Gene Names
CD70; CD27L; CD27LG; TNFSF7
Reactivity
Human
Synonyms
CD70 molecule; Human CD70 antigen; CD70 ELISA Kit; Human CD70 antigen (CD70) ELISA kit; CD27L; CD27LG; TNFSF7; CD27 ligand; CD70 antigen; Ki-24 antigen; surface antigen CD70; tumor necrosis factor (ligand) superfamily; member 7; tumor necrosis factor ligand superfamily; CD70 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human CD70. No significant cross-reactivity or interference between human CD70 and analogues was observed.
Sequence Length
193
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
0.039 ng /ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD70 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD70 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
970
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,118 Da
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 molecule
NCBI Official Symbol
CD70
NCBI Official Synonym Symbols
CD27L; CD27LG; TNFSF7
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name
CD70 antigen
Protein Family
UniProt Gene Name
CD70
UniProt Synonym Gene Names
CD27L; CD27LG; TNFSF7; CD27-L
UniProt Entry Name
CD70_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction

Research Articles on CD70

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Product Notes

The Human CD70 cd70 (Catalog #AAA911908) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA911908 ELISA Kit recognizes Human CD70. It is sometimes possible for the material contained within the vial of "CD70 molecule, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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