Mouse alanyl (membrane) aminopeptidase ELISA Kit | ANPEP elisa kit

Mouse Aminopeptidase N, ANPEP ELISA Kit

Cat. Num. AAA911187

• Gene Names: Anpep; Apn; AP-M; AP-N; Cd13; P150
• Reactivity: Mouse
• Synonyms: alanyl (membrane) aminopeptidase; Mouse Aminopeptidase N; ANPEP ELISA Kit; Mouse Aminopeptidase N (ANPEP) ELISA kit; APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M; aminopeptidase N; membrane alanine aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; ANPEP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ANPEP. No significant cross-reactivity or interference between mouse ANPEP and analogues was observed.
• Sequence Length: 966

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 39 mU/ml -2500 mU/ml.
• Sensitivity: The minimum detectable dose of mouse ANPEP is typically less than 9.7 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANPEP elisa kit

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 225637487
• NCBI GeneID: 16790
• NCBI Accession #: NP_032512.2
• NCBI GenBank Nucleotide #: NM_008486.2
• UniProt Accession #: P97449; Q91YH8; Q99K63
• Molecular Weight: 109,651 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: Anpep
• NCBI Official Synonym Symbols: Apn; AP-M; AP-N; Cd13; P150
• NCBI Protein Information: aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase
• UniProt Protein Name: Aminopeptidase N
• Protein Family: Aminopeptidase
• UniProt Gene Name: Anpep
• UniProt Synonym Gene Names: Lap-1; Lap1; AP-N; mAPN; AP-M
• UniProt Entry Name: AMPN_MOUSE

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Membrane protein, integral; Protease; Other Amino Acids Metabolism - glutathione; EC 3.4.11.2; Receptor, misc.

Cellular Component: extracellular space; membrane; lysosomal membrane; integral to membrane; ER-Golgi intermediate compartment; vesicle membrane; external side of plasma membrane

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; aminopeptidase activity; peptide binding

Biological Process: multicellular organismal development; aromatic compound metabolic process; angiogenesis; cell differentiation; proteolysis