Human transmembrane protein 27 ELISA Kit | TMEM27 elisa kit

Human Collectrin, TMEM27 ELISA Kit

Cat. Num. AAA909222

• Gene Names: TMEM27; NX17; NX-17
• Reactivity: Human
• Synonyms: transmembrane protein 27; Human Collectrin; TMEM27 ELISA Kit; Human Collectrin (TMEM27) ELISA kit; NX-17; NX17; 0610008J07Rik; collectrin; kidney-specific membrane protein; TMEM27 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TMEM27. No significant cross-reactivity or interference between human TMEM27 and analogues was observed.
• Sequence Length: 222

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 60 pg/ml -800 pg/ml.
• Sensitivity: The minimum detectable dose of human TMEM27 is typically less than 20 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human TMEM27 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TMEM27 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TMEM27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for TMEM27. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM27 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 15042969
• NCBI GeneID: 57393
• NCBI Accession #: NP_065716.1
• NCBI GenBank Nucleotide #: NM_020665.5
• UniProt Accession #: Q9HBJ8; Q6UW07; B2R9M1
• Molecular Weight: 25,235 Da
• NCBI Official Full Name: collectrin
• NCBI Official Synonym Full Names: transmembrane protein 27
• NCBI Official Symbol: TMEM27
• NCBI Official Synonym Symbols: NX17; NX-17
• NCBI Protein Information: collectrin; kidney-specific membrane protein
• UniProt Protein Name: Collectrin
• Protein Family: Collectrin
• UniProt Gene Name: TMEM27
• UniProt Synonym Gene Names: UNQ679/PRO1312
• UniProt Entry Name: TMM27_HUMAN

NCBI Description

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]

Uniprot Description

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: brush border membrane; cytoplasm; integral to membrane

Molecular Function: peptidyl-dipeptidase activity; protein binding; metallopeptidase activity

Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport