Transcriptional enhancer factor TEF-1 (TEAD1) Recombinant Protein | TEAD1 recombinant protein

Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1), partial

Cat. Num. AAA9018529

• Gene Names: TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transcriptional enhancer factor TEF-1 (TEAD1); Recombinant Human Transcriptional enhancer factor TEF-1 (TEAD1); partial; Transcriptional enhancer factor TEF-1(NTEF-1)(Protein GT-IIC)(TEA domain family member 1)(TEAD-1)(Transcription factor 13)(TCF-13); TEAD1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 210-426aa, Partial
• Sequence: SIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD

• Species: Homo sapiens (Human)
• Relevance: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Product Categories/Family for TEAD1 recombinant protein

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References

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.Sci. Signal. 3:RA3-RA3(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 296434319
• NCBI GeneID: 7003
• NCBI Accession #: NP_068780.2
• NCBI GenBank Nucleotide #: NM_021961.5
• UniProt Accession #: P28347
• Molecular Weight: 47,946 Da
• NCBI Official Full Name: transcriptional enhancer factor TEF-1
• NCBI Official Synonym Full Names: TEA domain family member 1 (SV40 transcriptional enhancer factor)
• NCBI Official Symbol: TEAD1
• NCBI Official Synonym Symbols: AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1
• NCBI Protein Information: transcriptional enhancer factor TEF-1; protein GT-IIC; transcription factor 13; transcriptional enhancer factor 1
• UniProt Protein Name: Transcriptional enhancer factor TEF-1
• Protein Family: Transcriptional enhancer factor
• UniProt Gene Name: TEAD1
• UniProt Synonym Gene Names: TCF13; TEF1; TEAD-1; TCF-13
• UniProt Entry Name: TEAD1_HUMAN

NCBI Description

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]

Uniprot Description

TEAD1: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Interacts with YAP1 and WWTR1/TAZ. Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression

Disease: Sveinsson Chorioretinal Atrophy