Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EBF1/COE1 Polyclonal Antibody | anti-EBF1 antibody
Goat anti-EBF1/COE1 Antibody
Gene Names
EBF1; EBF; COE1; OLF1; O/E-1
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
EBF1/COE1; Polyclonal Antibody; Goat anti-EBF1/COE1 Antibody; EBF1; early B-cell factor 1; COE1; EBF; O/E-1; OLF1; Collier; Olf and EBF transcription factor 1; early B-cell factor olfactory neuronal transcription factor 1; EBF1 antibody; early B-cell factor 1 antibody; COE1 antibody; EBF antibody; O/E-1 antibody; OLF1 antibody; Olf and EBF transcription factor 1 antibody; early B-cell factor olfactory neuronal transcription factor 1 antibody; EBF1 / COE1; anti-EBF1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HNQLPALANTSV
Sequence Length
591
Applicable Applications for anti-EBF1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx.75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.
Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing DYKDDDDK-EBF1.
Western Blot: Approx.75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml.
Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing DYKDDDDK-EBF1.
Immunogen
Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1.
Epitope
Internal region
Note
This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,059 Da
NCBI Official Full Name
transcription factor COE1 isoform 2
NCBI Official Synonym Full Names
early B-cell factor 1
NCBI Official Symbol
EBF1
NCBI Official Synonym Symbols
EBF; COE1; OLF1; O/E-1
NCBI Protein Information
transcription factor COE1
UniProt Protein Name
Transcription factor COE1
Protein Family
UniProt Gene Name
EBF1
UniProt Synonym Gene Names
COE1; EBF; O/E-1; OE-1
UniProt Entry Name
COE1_HUMAN
Uniprot Description
EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding; protein binding
Biological Process: multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on EBF1
Similar Products
Product Notes
The EBF1 ebf1 (Catalog #AAA421968) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EBF1/COE1 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EBF1/COE1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx.75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml. Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing DYKDDDDK-EBF1. Researchers should empirically determine the suitability of the EBF1 ebf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HNQLPALANT SV. It is sometimes possible for the material contained within the vial of "EBF1/COE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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