Rat Cytochrome P450 7A1 ELISA Kit | CYP7A1 elisa kit

Rat Cytochrome P450 7A1 ELISA Kit

Cat. Num. AAA8944

• Gene Names: CYP7A1; CP7A; CYP7; CYPVII
• Reactivity: Rat
• Synonyms: Cytochrome P450 7A1; Rat Cytochrome P450 7A1 ELISA Kit; CYP7A1 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat CYP7A1 and analogues was observed.
• Sequence Length: 504

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CYP7A1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CYP7A1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CYP7A1 and CYP7A1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CYP7A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CYP7A1 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CYP7A1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CYP7A1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CYP7A1 and CYP7A1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CYP7A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166295200
• NCBI GeneID: 1581
• NCBI Accession #: NP_000771.2
• NCBI GenBank Nucleotide #: NM_000780.3
• UniProt Accession #: P22680; P78454; Q3MIL8; Q7KZ19
• Molecular Weight: 57,661 Da
• NCBI Official Full Name: cholesterol 7-alpha-monooxygenase
• NCBI Official Synonym Full Names: cytochrome P450, family 7, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP7A1
• NCBI Official Synonym Symbols: CP7A; CYP7; CYPVII
• NCBI Protein Information: cholesterol 7-alpha-monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1
• UniProt Protein Name: Cholesterol 7-alpha-monooxygenase
• Protein Family: Cholesterol 7-alpha-monooxygenase
• UniProt Gene Name: CYP7A1
• UniProt Synonym Gene Names: CYP7
• UniProt Entry Name: CP7A1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

Uniprot Description

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Endoplasmic reticulum; EC 1.14.13.17; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 8q11-q12

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: cholesterol 7-alpha-monooxygenase activity; iron ion binding; heme binding

Biological Process: bile acid biosynthetic process; cholesterol homeostasis; bile acid metabolic process; xenobiotic metabolic process; cellular lipid metabolic process; cholesterol catabolic process; sterol metabolic process; transmembrane transport