Immunohistochemistry (IHC)
(Cl-Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Aggrecan (Cleaved-Asp369) antibody (#L0101).)
anti-Human, Rat Aggrecan Antibody | anti-ACAN antibody
Aggrecan (Cleaved-Asp369) Antibody
Gene Names
ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Aggrecan; Aggrecan (Cleaved-Asp369) Antibody; CSPG1; CSPGCP; MSK16; CHONDROITIN SULFATE PROTEOGLYCAN CORE PROTEIN 1; aggrecan 1 (chondroitin sulfate proteoglycan 1; large aggregating proteoglycan; antigen identified by monoclonal antibody A0122); aggrecan 1 isoform 1 precursor; aggrecan 1 isoform 2 precursor; cartilage-specific proteoglycan core protein; anti-ACAN antibody
Reactivity
Human, Rat
Specificity
Aggrecan (Cleaved-Asp369) antibody detects endogenous levels of fragment of activated Aggrecan resulting from cleavage adjacent to Asp369.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
2431
Applicable Applications for anti-ACAN antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
IHC: 1:50~1:100
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from human Aggrecan.
All Sites
Human: Asp369
Rat: Asp369
Rat: Asp369
Preparation and Storage
Store at-20 degree C for 1 year
Immunohistochemistry (IHC)
(Cl-Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Aggrecan (Cleaved-Asp369) antibody (#L0101).)
Immunohistochemistry (IHC)
(Cl-Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Aggrecan (Cleaved-Asp369) antibody (#L0101).)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using Aggrecan (Cleaved-Asp369) antibody (#L0101).Etoposide "Left Lane" + "Right lane" +Cl-peptide "Left Lane" - "Right lane" + )
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using Aggrecan (Cleaved-Asp369) antibody (#L0101).Etoposide "Left Lane" + "Right lane" +Cl-peptide "Left Lane" - "Right lane" + )
Product Categories/Family for anti-ACAN antibody
References
Ichiro Sekiya, J. Biol. Chem., Apr 2000; 275: 10738. Ying Liu, J. Biol. Chem., Nov 1997; 272: 29880. Russell T. Matthews, J. Neurosci., Sep 2002; 22: 7536. Amit Agrawal, Am J Physiol Cell Physiol, Apr 2007; 10.1152.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
250,372 Da
NCBI Official Full Name
aggrecan core protein isoform 1
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
ACAN
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
NCBI Protein Information
aggrecan core protein
UniProt Protein Name
Aggrecan core protein
Protein Family
UniProt Gene Name
ACAN
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1
NCBI Description
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Research Articles on ACAN
Similar Products
Product Notes
The ACAN acan (Catalog #AAA856610) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Aggrecan (Cleaved-Asp369) Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aggrecan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the ACAN acan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aggrecan, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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